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LSR Fortessa - red switch on the wall behind the monitors (Fig. A1). b. BD LSRFortessa X-20 Configuration Laser Detector Filter Mirror Dye 488nm (Blue) A 695/40 670LP PerCP, PerCP-Cy5.5 PerCP efluor 710 B 530/30 505LP FITC, GFP, CFSE, BB515 Alexa Fluor 488 C 488/10 - SSC 561nm (Yellow/Green) A 780/60 750LP PE-Cy7 B 670/30 http://stratedigm.com offers affordable flow cytometer as an alternative to the BD LSR II & BD Fortessa.Call( 408) 512-3901 to learn more about our flow cyto BD Fortessa HTS Procedure: 1) Remove the tube of water from the SIP and connect the HTS sample coupler. Leave the support arm to the side.

Our lab has the blue laser, red, yellow-green and violet. Experimental Design and Results Summary BD Fortessa X-20 – College of Medicine Cell Analysis Facility (COM-CAF) BD Fortessa X-20 Violet Laser (405 nm) Yellow Green Laser (561 nm) 1. Select Cytometer>CST. The cytometer disconnects from the BD FACSDiva interface and connects to the CS&T interface.

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Designed to be affordable and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. LSRFortessa X-20 SO 2B/3R/6V/5YG/2UV. For Research Use Only. Not for use in diagnostic or therapeutic procedures. BD LSRFortessa X-20 Flow Cytometer: Resolution Impact Matrix Resolution Impact Matrix (Color Coded) Secondary Fluorochrome Primary Fluorochrome FITC PerCP-Cy5.5 BV421 BV510 BV605 BV650 BV711 BV786 BUV395 BUV496 BUV661 BUV737 APC Alexa Fluor® … BD Fortessa X20 A Configuration and Fluorochromes Laser Diva Parameter Name Fluorochromes 488 nm Blue B 530/30 BB515, FITC, AF488, CFSE, GFP, TO-PRO-1 B 695/40 BB700, PerCP, PerCP-Cy5.5, PerCP-eFluor710, 7-AAD, PI 633 nm Red R 670/30 APC, AF633, AF647, TO-PRO-3 The BD LSR Fortessa™ X-20 cell analyzer delivers high-performance multicolor analysis detecting up to 18 parameters simultaneously with using 5 lasers: UV (355 nm) Violet (405 nm) Blue (488 nm) Y-G (561 nm) Red (640 nm) Fortessa X20 Flow Cytometer Quick guide Operation of the 5 laser Fortessa X20 BD Webinar CS&T System BD Compensation Guide… Analyser - Fortessa X20 BD LSRFortessa™ X-20 flow cytometer (Analyser) The BD LSRFortessa™ X-20 cell analyser delivers high performance multicolour analysis while maintaining a small bench space footprint.Our BD LSRFortessa X-20 is a 5 laser (Red 640nm, Yellow-green 561nm, Blue 488nm, Violet 405nm and UV 355) cytometer capable of analyzing up to 18 fluorescence parameters and 2 scatter The BD LSRFortessa™ X-20 cell analyzer delivers high performance multicolor analysis with the most compact footprint in its class. The BD LSRFortessa X-20 can be configured with up to 5 lasers, which enables the detection of up to 20 parameters simultaneously. The most common laser choices are blue, red, violet, yellow-green, and UV. null BD LSR Fortessa X-20 Description: The BD LSRFortessa X-20 cell analyzer provides power, performance, consistency and flexibility.

1.3. BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig. A1). b.
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The instrument has 4 independent laser lines (407, 488, 561 and 633 nm) and the possibility to detect up to 18 parameters with high sensitivity 2016-02-03 BD LSRFortessa X-20 or a BD FACSCelesta who want to learn the official procedures for maintenance as well as getting the knowledge necessary to make optimal use of the multiple options that these instruments offer. Dates and Registration To view available dates and register for a course, The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency.

In BD LSR Fortessa, BD LSR Fortessa X-20 and BD FACS Aria IIu we also run CS&T Beads ("Cytometer Setup and Tracking") which automatically verify the stability of the instrument.
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Home; Instruments; Scheduling and Calendars; Fee BD LSRFORTESSA X-20 QUICK GUIDE: START UP, QUALITY CONTROL, AND SHUT DOWN PROCEDURE 1.0 Start Up 1.1. Check that the sheath cube is full and the waste tank is empty. Add 250 ml of bleach to the empty waste tank. Check that the FACS Flow Cart is on.

The BD LSRFortessa X-20 is a premium flow cytometer that brings endless possibilities to multicolor cell analysis.